New Photos!

I did my first cell staining of cells treated with ascorbic acid
last night. All of these cells were plated on 3-1-05, and
stained/photographed on the evening for 3-4-05.
The original plating densites were:

1% FBS = 43.5 * 10^4 cells/mL
1% ITS = 15 * 10^4 cells /mL
99% OptM = 35.25 * 10^4 cells /mL

With the high FBS and OptM densities, cells became confluent almost
immediatly. All cells were plated in 50ug/mL ascorbic acid (100ul of
2000ug Asc/1ml mixed with 4mL media). It seems that high initial plating densities are key to plating cells succesfully at all. Cells which are plated at lower densities seem to divide very slowly, and most of them are lost when their media is changed for feeding because only a certain percentage seem be be adherent at any given time.
The orange ones are stained with safranin, and the blue ones are
stained with alcian blue. Cells were fixed for approx 15mins in 1:3
mix of 35% formaldehyde and 1x PBS. (I would have fixed for the normal 10mins but I got locked out of the cell culture room!!!! Luckily things seemed to have turned out fine.)
For the next batch I will try staining with safranin and ruby red,
and for the batch after that, maybe some fluorescent-antibody stain
(although from reading the protocol this looks like a long involved
process).